| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lin, Owen N (9)
|
NCECA |
42.30 |
41.02 |
-1.28 |
| 2 |
Zhang, Chuanqi (10)
|
NCYOTA |
42.42 |
44.86 |
2.44 |
| 3 |
Bullard, Andrew W (10)
|
NCECA |
|
45.28 |
- |
| 4 |
Johnson, Samuel T (10)
|
NCECA |
45.41 |
47.43 |
2.02 |
| 5 |
Bullard, Cameron E (10)
|
NCECA |
|
48.40 |
- |
| 6 |
Morris, Cameron J (10)
|
NCYOTA |
48.81 |
48.74 |
-0.07 |
| 7 |
Pittenger, Charles D (10)
|
NCYOTA |
55.63 |
50.02 |
-5.61 |
| 8 |
Zhang, Chuanxi (8)
|
NCYOTA |
42.39 |
50.28 |
7.89 |
| 9 |
Sha, Cody L (9)
|
NCYOTA |
50.48 |
54.47 |
3.99 |
| 9 |
Klein, Logan A (10)
|
NCECA |
50.03 |
54.47 |
4.44 |
| 11 |
Shanmuganathan, Akash A (9)
|
NCYOTA |
49.68 |
56.79 |
7.11 |
| 12 |
Bakelaar, Lucas A (9)
|
NCYOTA |
1:01.27 |
1:02.20 |
0.93 |
| 13 |
Murray, Angus (9)
|
NCBBS |
|
1:02.39 |
- |
| 14 |
Croom, Jack R (9)
|
NCYOTA |
1:09.00 |
1:04.95 |
-4.05 |
| 15 |
Lines, Oliver D (10)
|
NCYOTA |
|
1:10.35 |
- |
| 16 |
Jewett, Matthew T (9)
|
NCYOTA |
|
1:11.39 |
- |
| 17 |
Lehman, Kaelan J (9)
|
NCYOTA |
|
1:15.32 |
- |
| 18 |
Schwartz, Elijah J (8)
|
NCECA |
|
1:27.20 |
- |
|
Green, Xavier D (6)
|
NCYOTA |
|
DQ |
- |
|
Siska, Ethan S (7)
|
NCECA |
|
DQ |
- |
|
Stuart, William E (8)
|
NCYOTA |
|
DQ |
- |
|
DeMacy, Brendan * (10)
|
NCYOTA |
48.69 |
NS |
- |
|
Mehra, Ryan (10)
|
NCYOTA |
1:25.85 |
NS |
- |